Plates were shaken continuously at medium speed, and temperature was kept at 37°C. Spectramax M5 plate reader, 5 modes, 200-100 nm Abs range, Abs, FL, Lumi, TRF and FP, Monochromator in 1 nm increments, includes validation and warranty. Catalog number: 165306. " SpectraMax M5 micro plate reader is a multi mode plate reader (FLU, LUMN . 6-, 12-, 24-, 48-, 96- and 384-well plates. 1. Select Start>Control panel>Device Manager. SpectraMax M3, M4, M5, and M5 Multi-Mode Microplate Readers Optimizing Fluorescence Polarization Assays Fluorescence polarization for SpectraMax M5 and M5 readers may only be read from the top of a microplate. wavelength and 595 nm emission wavelength by Spectramax M5 plate reader (Molecular Devices). Release of free AMC by proteolytic activity was monitored on Molecular Devices M5 plate reader with excitation and emission wavelengths of 360 and 460 nm, respectively. 9000-0756. Linear, Orbital Slow, and Orbital Fast. Cell Analysis. Viability Assay reagent (Promega). 40266 Email: drugdiscoverytech@lifetech. The supernatants collected in Subheading 3. The IC50 was calculated using the DRC package in the R software. Cells were seeded at a density of 1,000 cells/well (A549: 500 cells/well). to meet all your research needs. To quantify the cell apoptotic degree, the harvested cells were stained with. 1% Tween-20 and bound secondary was detected using Ultra TMB (Thermo Fisher); signals were read on a Molecular Devices Spectra Max M5. One unit of hexokinase is the amount of enzyme that will generate 1. If the computer is connected to the Internet: Select Search Automatically for updated driver software. Spend a little more and you can get an FTDI-based USB cable. SpectraMax® iD5 Multi-Mode Microplate Reader - Five-Mode Hybrid Microplate Reader with Automatic NFC Filter Identification and Western Blot Capability, Molecular Devices. The solution was shaken at 37°C and 1200 rpm for 3 days. ELISAs were carried out according to the manufacturer’s instructions for IL-1β (BD Biosciences, San Diego, CA), TNFα, MIP-1α, and MCP-1 (R&D Systems, Minneapolis, MN) and quantified using a SpectraMax M5 plate reader (Molecular Devices, Sunnyvale, CA). The following components are located on the back panel of the SpectraMax and VersaMax readers: • Power switch: Step 4: Incubate at room temperature for 1 hour, protected from light. Test Plates for absorbance and fluorescence intensity provide a complete traceable solution for validating optical performance of the SpectraMax M2 reader, automatically. 314 low lamp level warning. The % PEG midpt and apparent solubility values (thermodynamic activity) were calculated as described previously. 4: Test tube cover. 0200-0729. Luminescence was detected using the Bright-Glo Luciferase Assay System (Promega) on a SpectraMax M5 plate reader (Molecular Devices). Results: In the presence of Cliradex ®, Johnson’s® Baby Shampoo, OCuSOFT Lid Scrub®were read using SpectraMax M5 plate reader (Molecular Devices, San Jose, CA, USA) with excitation of 380 nm and emission of 460 nm. Immunohistochemistry Four μm kidney sections were heated at 65 °C for 1 hr and deparaffinized in xylene, followed by rehydration in decreasing concentrations of ethanol (two washes in 100% ethanol, two washes in 95%, one wash in 70%, one wash in 50%,. T o enable accurate quantita-tion, initially standard curves were plotted f or both Pi and. Reader Documentation Version No. : 8 Oct 12 Page 7 of 10 Setup Guide on the Molecular Devices SpectraMax® M5/M5e Microplate Reader Have a question? Contact our Technical Support Team NA: 800-955-6288 or INTL: 760-603-7200 ext. The GloMax® Explorer is a high-performance multimode plate reader that allows you to get up and running quickly, generating the data you need from your experiments. Avantor®, a Fortune 500 company, is a leading global provider of mission-critical products and services to customers in the biopharma, healthcare, education & government, and advanced technologies & applied materials industries. For SpectraMax L or LMax II, LMax II 384 - no adapter used. The plates were centrifuged at the end of incubation, and fluorescent signals in supernatants were measured using a SpectraMax™ M5 microplate reader, with excitation at 485 nm and emission at 520 nm. Eis inhibitors were dissolved in Tris-HCl (50 mM, pH 8. The SpectraMax® ABS Plus Microplate Reader can run . Supersedes 1. 2016 Aug 2; 7(31): 50027–50042. The SpectraMax M5 features a triple-mode cuvette port and a single-read command capability for experiments requiring multiple detection parameters. EUROPE +44-118-944-8000 Mon to Fri 8am - 5pm GMT. Brand: Molecular Devices | Category: Laboratory Equipment | Size: 0. If the computer is connected to the Internet: Select Search Automatically for. 40266 Email: [email protected] fluorescence intensity was determined using a fluorescent plate reader (SpectraMax M5 plate reader) set at an excitation wavelength of 485 nm and an emission wavelength of 530 nm. SpectraMax M5 is a fantastic plate reader - easily my #2 behind PerkinElmer (if you can afford them). With optimized reagents, option to operate the readers. The solids content was measured using a MA35 Moisture Analyzer (Sartorius AG). Category 2 and 3 microplate readers use gradient heating as well. The empty LNP matrix solution was made by mixing 300 μL of empty LNP solution with 45 mL diluent. Our multi-mode plate readers provide great flexibility and include absorbance, fluorescence and luminescence with configurable options for fluorescence polarization (FP), time-resolved fluorescence (TRF), FRET, and AlphaScreen. Product Overview. The concentration of MMP-9 was measured using R&D System’s ELISA Kit (Minneapolis, MN) on a Spectramax M5 plate reader equipped with SoftmaxPro v5. 5. 1 software to identify the IC50. [1] AnkerMake M5 is capable of printing at a speed of 500 mm/s and acceleration of 5,000 mm/s² with the new Fast Mode upgrade. Monthly Plate Verification Check (Absorbance Validation Plate) Note: Monthly Plate Verification Check is maintained by Quality Control. 51 L/g). GraphPad Prism was used to process the data and calculate the Km and Vmax values. Purified fluorescent protein was diluted 15-fold into 0. 5. Accordingly there are different configurations possible. 18632/oncotarget. Help the planet as you cruise along at 5X speed. 9. The plate was incubated at 37 °C for 30 min. For a Price List, please call (858) 646-3100 ext. 0 and room temperature. Detection modes are available in UV-Vis absorbance, fluorescence, and. This microplate reader handles Absorbance, Fluorescence Intensity, luminescence, Time resolved fluorescence, fluorescence polarization. Results were expressed as absorbance units. Fe standards require only about 30 minutes but can be kept in 1x working solution for at least a week withoutAfter 15 min, the absorbance at 550 nm was recorded using a Molecular Devices SpectraMax M5 plate reader. Description Figure 1. edu, 814-863-3503. The peptide concentration was. Furthermore, VersaMax system is reliable, which means more uptime and lower long-term cost of ownership. Product Type. The user must ensure that the meniscus is comfortably above the light beam in standard cuvettes and that the sample chamber in a microcuvette is aligned properly with the beam. The plate can be shaken (icon right of thermometer, and see automix option in setup). The SpectraMax® M3 Multi-Mode Microplate Reader features dual monochromatic optics to allow the widest range of applications to be utilized for bioresearch procedures, without the need to change filters. Cell Titer Glo reagent (Promega) was then added to each well, and luminescence (RLU) was measured on a SpectraMax M5 plate reader (Molecular Devices). DH5α and BL21 (DE3) chemically competent E. The software is easy to use and contains settings for hundreds of commonly used assays, such as BCA and MTT. We therefore gathered salient. Our lab has a BMG labtech Pherastar FS, it is easy to use and we have never had any issue with it. The SpectraMax M5 features a triple-mode cuvette port and a single-read command capability for experiments requiring multiple detection parameters. Lucifer yellow was measured using an M5 plate reader (Molecular Probes) at an excitation wavelength of 485 nm and an emission wavelength of 530 nm. 4 fmol/well, 384-well plate)−bottom Reading Speed (Kinetic) 96 wells: 11 s 384 wells: 22 s Luminescence Wavelength Range 300−700 nm Dynamic Range > 6 decades Sensitivity. 2. Download a PDF version of the SpectraTest validation package. LbL films containing TRITC- or FITC-labeled PAA are degraded by DTT and monitored by a fluorometer (SpectraMax M5 Plate Reader, Molecular Devices). Microplate Readers; SoftMax Pro Software; Microplate Stacker and Washer; Cellular Imaging Systems; Clone Screening; FLIPR; AXON Patch-Clamp; Assay Kits. Representative fluorescence images of the cell-containing scaffolds and well plate are shown in Fig. 6 for further experiment, so I'm confused which i would choose for OD value determination. 2. In general, reading fluorescence from the top is more sensitive than reading from the bottom. Fast protein liquid chromatography (FPLC) was performed as the last protein purification step on a Bio-Rad BioLogic DuoFlow using a HighPrep TM 26/60 Sephacryl TM S-200 High Resolution column. For example, microplates containing different samp les can be read using the same orThe 526/470 ratios for the standards were plotted against the standard concentrations. Devices’ StakMax® Microplate Stacker in a matter of minutes and . 10 μL of each SCGal-Ura and SC-Ura culture were then transferred to 1 mL fresh SC-URA and allowed to grow to saturation overnight. The fluorometric performance of theAbsorbance is read with a SpectraMax M5 plate reader. The dual monochromator optics allow the widest range of applications to be utilized for bioresearch and drug discovery applications, all without the need to change. Optimal timing of signal detection occurred 15 min after reaction initiation with the kit substrates. For example, microplates containing different samp les can be read using the same orChoose the desired plate type, using the upper dropdown menu to choose plate format (96 or 384 wells) and the "Select Specific" menu to choose the specific plate type. 5. 5. The decrease in absorbance at 340 nm was analyzed for the 200 s of the reaction, which corresponds to the initial rate of the reaction. PURPOSE. 2. Reactions were performed on a 384-well plate, with release of 6-FAM dye monitored by fluorescence at 517 nm using a SpectraMax M5 plate reader (Molecular Devices) with excitation at 495 nm for 60. Guidelines to using the Qubit Flourimeter. Perkin Elmer Victor X4 Multimode Plate Microplate Reader 2030-0040 with PC. Use form “26003-02: Molecular Devices Plate Reader Plate Calibration Form” to record material and equipment information. com 10. SpectraMax M5 Plate Reader: 901F: Nanodrop 2001: BioRad ChemiDoc gel documentation (old) Bio-Rad T100Tm Thermal Cycler : Bio-Rad CFX ConnectTM Real-Time System : Location. Readings were taken every 30 minutes. Protocol for Qubit DNA HS Assay. Dear Nelly, In a micro plate the fluorescence emission optics are very different from those in a single cell because of obstruction, reabsorption etc. In the M2, M5 and Plus 384, the light. To the OP, if you are familiar with VAG-COM, you know that RS232-based VAG-COM cables are in the $14 range on eBay. The SpectraMax Quant dsDNA Assay Kits provide pre-optimized, complete solutions that are sensitive, accurate, and easy to use. We use Spectramax M5 microplate reader in our lab. To monitor the aggregation kinetics, 10 μM ThT was added to a small aliquot (300 μL) of the mixture, and the fluorescence signal (λ ex = 444 nm, λ em = 485 nm) was monitored on a Spectramax M5 plate reader (Molecular Devices). Louis, MO) dissolved in PBS was injected over 30 seconds into the tail vein of a mouseIn bacteria, natural transposon mobilization can drive adaptive genomic rearrangements. Clotting and lysis were monitored in a Spectramax M5 plate reader (Molecular Devices, Berkshire, United Kingdom) at 340nm (plasma) or 405nm (fibrinogen) and analyzed using online apps to determine times to 50% lysis. g. 1. In my opinion one the best plate readers which I used till now is Molecular Devices SpectraMax M5 Plate reader. Incubation. This unit must survive. High-performance luminescence, fluorescence, UV-Visible absorbance, BRET and FRET, two-color filtered luminescence and kinetic measurement capabilities. Immunoblotting. 6 for further experiment, so I'm confused which i would choose for OD value determination. Published online 2016 Jul 13. 5: Schematic of the back panel of a reader. The colorimetric reaction was stopped with 2 M sulfuric acid, and absorbance was measured at 450 nm using a SpectraMax M5 plate reader (Molecular Devices). 1. Discovery of CRBN as a target of thalidomide: a breakthrough for progress in the development of protein degraders. The Back Panel Figure 1. There is currently no charge for using this plate reader. 5 hours after an initial 10-minute incubation period. Published Dec 16, 2020 | Updated Dec 16, 2020. ThT fluorescence was measured at 444 and. I have a 2002 M5 and my medium-deluxe Harbor Freight reader doesn't talk to the BMW whatsoever. Concentrations of other cytokines were determined by using custom MSD kits and a QuickPlex SQ120 plate reader (Meso Scale Discovery,. 305 reference level saturation. The plate was then incubated at 37°C for 30 min to allow complement mediated lysis of the cells to take place. Table of Contents. The absorbance was measured using Molecular Devices Spectramax M5 plate reader with the software Softmaxpro at 570 and 650 nm. 25 fmol/well, 384-well plate)−top Fluorescein 4 pM (0. 2016 Aug 2; 7(31): 50027–50042. Clear Filters. 1. After the plate is read, data will appear in the plate section: 11. PMC full text: Oncotarget. 3 Kg) Regulatory Regulatory CE and TUV marked. 6. Representative fluorescence images of the cell-containing scaffolds and well plate are shown in Fig. The absorbance at a wavelength of 540 nm was quantified using a Spectramax M5 plate-reader (Molecular Devices) to determine the amount of gamma glutamyl hydroxamate, indicative of active enzyme. 5. After mixing all the components, the absorbance of the mixture was continuously measured at 475 nm at 25°C using a SpectraMax M5 plate reader (Molecular Devices, Sunnyvale, CA, USA). A standard curve was plotted using various numbers of live cells and used to calculate the bacterial viability (%) in the test samples. This is a result of the light being attenuated and scattered by the. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. For the Evans blue (EB) dye extravasation test, 3,8. After addition of the freshly diluted substrate (50 μL/well, from Promega), bioluminescence was immediately measured on a SpectraMax M5 plate reader (Molecular Devices) using the luminescence mode. The plate was incubated for 4 h and the absorbance of the solubilized formazan was measured on a Molecular Devices Spectramax M5 plate reader at 570 nm. SpectraMax L Microplate Reader is a sensitive luminometer with programmable injector options that measures flash and glow luminescence assays, including dual luciferase reporter gene, G protein-coupled receptor (GPCR) in 96- and 384-well plates. You can also interpret your results using integrated data analysis software. Extreme caution is recommended when you use organic solvents. The samples were loaded into a rectangular quartz cell with the light path length of 1 mm. The wavelength required to excite the sample can be selected using either a filter of a specific wavelength or a monochromator tuned to the required. com 10. The plates are optimized for fluorescence and luminescence in cell culture and microscopic applications. Colorimetric detection of crystal violet released into the supernatant, which is proportional to the number of attached cells, was determined using a SpectraMax M5 plate reader (Molecular Devices). Reactions on 384-well plates (Corning) were monitored by fluorescence (emission 545 nm) using a SpectraMax M5 plate reader (Molecular Devices), with excitation at 274 nm, for 20 min at 2-min. Standard features include a cuvette port, spectral scanning in 1 nm increments, and up to six wavelengths per read. 01–10 fmol/well (0. 5. Enter Sample IDs (HPV-Type, Sample Description and Data Reference when applicable), Dilution Factors, and background information into the template. The SpectraMax®M3, M4, M5, and M5eMicroplate Readers are a series of dual-monochromator, multidetection, multi-mode instruments with a triple-mode cuvette port. Detection modalities. 33 Hz acquisition rate (one acquisition every 2 sec plus 1 sec for mixing in between each acquisition), using 495 and 585 nm. Data were expressed as % control =. There is currently no charge for using this plate reader. edu The content of this site is published by the site owner(s) and is not a statement of advice, opinion, or information pertaining to The Ohio State University. In the closed conformation, the regulatory SH3 and SH2 domains pack against the back of the kinase domain, providing allosteric control of kinase activity. Most papers they cultured bacteria to OD600>0. SA-β-gal assay. 사용이 편리하고 견고한 생명과학 연구, 신약 개발, 개발, Bioassay용 assay. 1, and the color is formed by the reaction in the microtiter plate. 0 adjusted at rt containing 10% v/v DMSO) (100 μL) and a 2- or 5-fold dilution was. edu, 814-863-3503. Empirical Phase. SpectraMax Injector Cartridge with SmartInject™ Technology. SoftMax Pro Software can collect data from one or more microplates or cuvettes and store it in a single data file, using the same or different instrument settings for different microplates or cuvettes. 9000-0756. Monocytes were seeded in 24 or 96 well plates at 5 × 10 5 cells/mL and differentiated to macrophages for 7 days in X-VIVO 10 (Lonza) medium supplemented with 1% human serum as previously described . Windows® 8. Reader Documentation Version No. Published Dec 16, 2020 | Updated Dec 16, 2020. 흡광, 형광, 발광 중 하나의 측정 모드만 이용할 수. fluorescence intensity, time-resolved fluorescence (M4, M5, M5 e models only) or wavelength-selectable luminescence modes, and 400–750 nm for readings in fluorescence polarization mode (M5, M5e models only). To eliminate the differences of TCR expression levels among different JRT cell lines, relative luminescence unit (RLU) was normalized to the condition with Human T-Activator DynaBeads at 2:1 bead-to-cell ratio. Gemini XPS and EM Microplate Readers with dual monochromator fluorometers provide a flexible environment to determine the optimal excitation and emission settings for fluorescence intensity assays. Other features. Cells were collected and fixed with precooled 95 % ethanol at 4 ºC for 12 h. After cell attachment, culture media was replaced with F-12 media containing 1% FBS for overnight incubation. 313 reference gain check fail. After this time period, the plate was read for fluorescence intensity at ex/em wavelengths of 532/590 nm, on a SpectraMax M5 plate reader. Activation of reporter constructs was monitored using the Dual-Glo Luciferase assay (Promega) and measured in a Spectramax M5 plate reader. The SpectraMax® M Series Multi-Mode Microplate Readers are modular and upgradeable with a wide range of high performance capabilities ideal for life science research and drug discovery screening. 단일 튜브 디스펜싱에서 전체 96 및 384 well 플레이트세척까지 간소화된 Microplate 세척입니다. 5 pM (0. The Molecular Devices SpectraMax® M5/M5e Microplate Reader was tested for compatibility with Life Technologies GeneBLAzer® assays. SpectraMax M5 plate reader (Molecular Devices, San Jose, CA) was used. Select the Driver tab. Our comprehensive multipoint inspection and testing ensures each microplate reader or washer meets our product operational specifications. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. Plate section without PathCheck applied. 2000) supplemented with each chemical com-pound with the final concentration of 10 mmol l-1. 10569C 2 C 12 cells were treated exactly as described for free [Mg 2+] determination, except that MgG was replaced by 5 μM safranine O. Copia stocks a full line of used microplate readers. Enter Sample IDs (HPV-Type, Sample Description and Data Reference when applicable), Dilution Factors, and background information into the template. Endothelial permeability was also measured in vitro, using a transwell assay. SpectraMax Injector Cartridge with SmartInject™ Technology. 9. The 2018 M5 is powered by a 600-hp twin-turbocharged 4. 2. Please contact Tatiana to reserve the plate reader time: [email protected] or . Rating: 5. To determine the DAR, STRO-002 in plasma samples were captured using Streptavidin Mag Sepharose Beads (GE Healthcare) coated with biotinylated. Here, we build on this capability and develop an inducible, self‐propagating transposon platform for continuous genome‐wide mutagenesis and the dynamic. The average 526/470 ratio for the sample aliquots. 72 MB. After 48-h growth at 25 C with agitation, the optical density was recorded using a SpectraMax M5 plate reader (Molecular Devices, Sunnyvale, CA, USA). Review date: 07 Nov 2017 | SpectraMax® M5 Multi-Mode Microplate Reader. RNA-Seq . 6. View the LabX ads below to find Molecular Devices SpectraMAX plate readers for sale and. The SpectraMax iD5 reader uses the HTRF detection system, which includes an Enhanced TRF Module and filters for excitation and emission. Page 1 of 8 . Molecular devices spectramax™ m5 microplate reader w/ warranty (svcSpectramax® m5/m5e multimode plate reader, molecular devices Molecular devices announces next-generation spectramax minimax 300Microplate spectramax m5 molecular reader devices contract warranty svc available labx biotech laboratory readers science. Highly selective for dsDNA over ssDNA or RNA. 2. Turn on Plate Reader and wait for it to complete its start-up routine. Fuchs1,2,3* 1 Department of Biology, Tufts University, Medford, MA 02155, USA 2 Institute for Protein Innovation, Boston, MA 02115, USA 3 Program in Cellular and Molecular Medicine,. 4. 4 software (Molecular Devices, San Jose, CA, USA). The modular platform allows upgrading to expand functionality as your laboratory workflows change. 0200-0729. Plate Reader (3) Name Price; EnSpire Flexstation 3 SpectraMax M5 Plate. Quantitative PCR RNA was collected in duplicate from 1 × 10 6 cells lysed in TRIzol Reagent (Invitrogen, 15596018) after. Most papers they cultured bacteria to OD600>0. Fluorescence microplate readers are multimode spectrophotometers with the capability to detect and measure fluorescence emitted by analytes in a multi-well sample format. Products. Apoptosis was measured by annexin V–fluorescein isothiocyanate (FITC)/7-amino-actinomycin D (7-AAD) labeling followed by fluorescence flow cytometry as previously described. Typically the system can detect absorbance, luminescence, fluorescence,. Description. , Sunnyvale, CA, USA) at the excitation wavelength of 488 nm for carboxy-H2DCFDA and 505 nm for DHE and MitoSOX ™. Category 2 and 3 microplate readers use gradient heating as well. Molecular Devices SpectraMax M5 Multi-mode Plate Reader 6. Products : FlexStation 3 reader, SpectraMax M5/M5e reader, SpectraMax M2/M2e reader. 6), turn on the M5 Plate Reader and open the “BCA Template” file 9. ameters by UV-Vis assays was done on a multimode SpectraMax M5 plate reader by using 96-well plates (Fisher Scientific). The plates were washed thrice with kit wash buffer; 150 µl kit substrate was then added to each well and the plate incubated 30 min at room temperature (with shaking). The reduced readings obtained from the plate reader were analyzed using PRISM 6. Molecular Devices LLC temperature controlled molecular devices m5 plate reader Temperature Controlled Molecular Devices M5 Plate Reader, supplied by Molecular Devices LLC, used in various techniques. S. 2. The inhibitory rate of ingredients was calculated as follows: Inhibition (%) = (FI of control – FI of treatment) / FI of control × 100. After 30 min, the plate was washed with PBS 0. Find regional support. 3). Kojic acid was used as a positive control. A solution for low volume applications. Plates were placed on a mi-croplate shaker for 2 min, then incubated at room temperature for 10 min to stabilize luminescent signal. 9. Multimode microplate reader equipped with latest detections like fluorescence, absorbance etc. The Chemistry Department is housed in an air-conditioned building, sharing space with the Departments of Biology and Molecular Biology & Biochemistry. Patented Hybrid Technology delivers the sensitivity of filters with the flexibility of monochromators. 0 . The software is simple to use and set up protocols. The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. Page 1 of 8 . Blue, kinase (no inhibitor); green, no kinase. 025 fmol/well, 384-well plate) Quad monochromator: Fluorescein 2. Also refer to this Multimode Reader Comparison Chart. Chirag considers the SpectraMax M5 a multi-purpose reader that. SpectraMax Readers have the most complete level of product validation and compliance. 25 pM (0. IC 50 values were determined on a multimode SpectraMax M5 plate reader using 96-well plates (Thermo Fisher Scientific) by monitoring absorbance at 412 nm taking measurements every 30 s for 20 min. Varian Inova 500 MHz three-channel Z-axis PFG NMR. Gemini XPS & EM readers measure fluorescence on a variety of sample formats from 6- to 384-well. Fluorescence Microplate Reader Comparison Chart. 9000-0756. Published online 2016 Jul 13. All values reported represent the mean of at least three independent experiments. AB-0558) and immediately read for luminescent signal on a SpectraMax M5 plate reader. Spectrofluorometers or plate readers with fluorescence detection typically offer greater sensitivity and a wider dynamic range compared to absorbance detection. Our luminescence plate readers, also referred to as luminometers are ideal for measuring a wide range of assays including dual luciferase reporter gene, G protein-coupled receptor (GPCR) via aequorin, bioluminescence resonance energy transfer. Vials were coded so that the person running the samples was blinded to the contents. S6. A microplate reader with fluorescence intensity (FI) detection uses a light source, usually a Xenon flash lamp or LED, to excite a fluorophore (fluorescent molecule) at a particular wavelength. Table of Contents. 2. That explains the lower values on the LmaxII384 plate reader. Multiskan Verification Plate, 8. To detect the luminescent signals, 100 μl of each dilution was added into a 96-well plate before adding 50 μl of LumiGLO Peroxidase Chemiluminescent substrate (KPL, 54-71-00) to measure luminescent intensity by a SPECTRAmax M5 plate reader. Uncontrolled Copy for Training and Reference Purposes Only . The purpose of this procedure is to set instructions in the proper use and handling of the Molecular Devices M5 Plate Reader. Because many reagents can be fluorescently labeled, spectrofluorometers are used to detect many biological and chemical processes. RNA for the RNA-Seq experiment was obtained exactly as described previously for the quantitative RT-PCR. It provides ready-to-run protocols, analysis algorithms, and 21 different curve fit options. The optical density (OD) at 450 nm was recorded by SpectraMax M5 plate reader (Molecular Devices, CA). The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF), and tunable fluorescence polarization (FP) read modesRobust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter. Developed with Promega reagents to provide a. About. This procedure is illustrated in Fig. CHO-K1 cells were transfected with luciferase. To determine whether each drug had proved. Remove plate cover. They provide absorbance, fluorescence, glow luminescence, and other read modes for programmable endpoint, kinetic, multiple-wavelength linear scan and area scan measurements. 25 fmol/well, 384-well plate)−top Fluorescein 4 pM (0. 95. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. 4: Test tube cover. Fluorescence Microplate Readers. HSL_EQ_004: Use and Maintenance of a BioTek Plate Washer in the HPV Serology Laboratory 3. 5. SoftMax Pro Software is an industry-leading acquisition and analysis software with over 160 ready-to-run protocols, powerful data analysis, and a range of data output options. 2. com 10. Cytation 5 combines automated microscopy and conventional microplate detection in a configurable, upgradable platform. 0. Models for In. 2. Microplate Reader Shelf. SOP Title: Use and Maintenance of a Molecular Devices M5 Plate Reader Document ID: HSL_EQ_005 Version 2. 2M posts 124. plate loading adapter and a choice of magazines for 20, 40 or 50 SBS-standard 96- and 384-well microplates. 5. The SpectraMax iD5 User Guide is a comprehensive document that provides detailed instructions on how to set up, operate, and maintain the SpectraMax iD5 Multi-Mode Microplate Reader. In general, reading fluorescence from the top is more sensitive than reading from the bottom. The luminescent signal was recorded on a SpectraMax M5 plate reader (Molecular Dy-namics). [4] The IC 50 value of each drug was calculated by the Logit approach. 0 mM of NADH per min at pH 8. The patented Hybrid Technology offers flexibility and sensitivity across a broad range of applications in the clinical laboratory. cerevisiae transcription factor Azf1p Taylor Stewart1, Benjamin E. Plate stacker and robot integration The Gemini XPS Reader can be integrated with Molecular Devices’ StakMax® Microplate Stacker in a matter of minutes and begin reading microplates with seven mouse clicks. For a higher degreeTime Resolved Fluorescence: Solid white plates for top reads and white plates with clear bottoms for bottom reads. Fluorescence, brightfield, color brightfield, and phase contrast imaging are available with the automated cell imager. 5. A. Lucifer yellow concentrations in the donor and acceptor wells were measured in the last of this incubation. This five-mode microplate reader with automatic filter identification and western blot capability is the complete. the contents were carefully mixed by pipetting up and down. Monitor reader performance on demand to ensure data quality is not compromised between PM/OQ. The FilterMax™ F3 and F5 Multi-Mode Microplate Readers offer a wide set of features at an affordable price-to-performance ratio. After the plate is read, data will appear in the plate section: 11. Users can choose to process all plates within the magazine or select a specific number. Wolfe1, and Stephen M. On the day of the assay, the plate was equilibrated to room temperature, and then 100 μL reconstituted Steady-Luc working solution containing D-Luciferin was added to each well. fluorescence intensity, time-resolved fluorescence (M4, M5, M5 e models only) or wavelength-selectable luminescence modes, and 400 750 nm for readings in fluorescence polarization mode (M5, M5 e models only). CD spectra were recorded using a Jasco-715 circular dichroism spectrophotometer at 20 °C. Information on how to reserve the plate readerMolecular Devices SpectraMax M5 User Manual (86 pages) Multi-Mode Microplate Readers. The system also gives users the flexibility to choose wavelengths between 200 nm and 1000 nm. Third this recommendation. There is currently no charge for using this plate reader. Turn the reader on and secure the USB cable. Open the program, and set the temperature to 37 °C for preheating. Options include top and bottom detection, temperature control and reagent injector.